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The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are...
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The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region
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Title
The
capsule
polysaccharide
structure
and
biogenesis
for
non-O1
Vibrio
cholerae
NRT36S
:
genes
are
embedded
in the
LPS
region
Author
Bush
,
C
Allen
Co-Author(s)
Chen
,
Yuansha
;
Bystricky
,
Peter
;
Adeyeye
,
Jacob
;
Panigrahi
,
Pinaki
;
Ali
,
Afsar
;
Johnson
,
Judith
A;
Morris
,
JG
;
Stine
,
OC
UMBC Affiliation
Chemical and Biochemical Engineering
Date Digitized
2013-01-02
Document Type
research
article
Peer reviewed
yes
Language
en
Keywords
capsular
(CPS)
polysaccharide
,
LPS
biosynthesis
genes
,
V
.
cholerae
,
surface
polysaccharide
,
biogenesis
Abstract
Background
: In
V
.
cholerae
, the
biogenesis
of
capsule
polysaccharide
is
poorly
understood
. The
elucidation
of
capsule
structure
and
biogenesis
is
critical
to
understanding
the
evolution
of
surface
polysaccharide
and the
internal
relationship
between
the
capsule
and
LPS
in this
species
.
V
.
cholerae
serogroup
O31
NRT36S
, a
human
pathogen
that
produces
a
heat-stable
enterotoxin
(NAG-ST)
,
is
encapsulated
. Here,
we
report
the
covalent
structure
and
studies
of the
biogenesis
of the
capsule
in
V
.
cholerae
NRT36S
.
Results
: The
structure
of the
capsular
(CPS)
polysaccharide
was
determined
by
high
resolution
NMR
spectroscopy
and
shown
to be a
complex
structure
with
four
residues
in the
repeating
subunit
. The
gene
cluster
of
capsule
biogenesis
was
identified
by
transposon
mutagenesis
combined
with
whole
genome
sequencing
data
(GenBank
accession
DQ915177)
. The
capsule
gene
cluster
shared
the
same
genetic
locus
as that of the
O-antigen
of
lipopolysaccharide
(LPS)
biogenesis
gene
cluster
.
Other
than
V
.
cholerae
O139
, this
is
the
first
V
.
cholerae
CPS
for
which
a
structure
has been
fully
elucidated
and the
genetic
locus
responsible
for
biosynthesis
identified
.
Conclusion
: The
co-location
of
CPS
and
LPS
biosynthesis
genes
was
unexpected
, and would
provide
a
mechanism
for
simultaneous
emergence
of
new
O
and
K
antigens
in a
single
strain
. This, in
turn
,
may
be a
key
element
for
V
.
cholerae
to
evolve
new
strains
that
can
escape
immunologic
detection
by
host
populations
.
Bibliographic Citation
Bush, C. A., et al. "The Capsule Polysaccharide Structure and Biogenesis for Non-O1 Vibrio Cholerae NRT36S: Genes Are Embedded in the LPS Region." BMC Microbiology., 15 Mar. 2007. 02 Jan. 2013 <
Date Copyrighted
2007
Date Published
2007-03-15
Original Publisher
BMC
Microbiology
Collection
Faculty
Publications
Rights
This
item
may
be
protected
under
Title
17
of the
U.S
.
Copyright
Law
.
It
is
made
available
by
UMBC
for
non-commercial
research
and
education
. For
permission
to
publish
or
reproduce
,
please
contact
the
author
.
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